Journal: International Journal of Molecular Sciences

Article Title: Phenolic Compounds with Antioxidant Properties from Canola Meal Extracts Inhibit Adipogenesis

doi: 10.3390/ijms21010001

Figure Lengend Snippet: ( A ) Immunofluorescence staining of PPARγ in CME treated mesenchymal stem cells (CH310T1/2) visualized at 10 µm: ( UN ) undifferentiated cells—negative control; ( DC ) differentiated cells—positive control. Differentiated cells treated with CMEs: Acetone extract ( AE ); methanol extract ( ME ); butanol extract ( BE ); ethanol extract ( EE ); hexane extract ( HE ); chloroform extract ( CE ); and water extract ( WE ). Captured images with no number labels are cells stained with fluorescein isothiocyanate (FITC). Images labeled with “1” show cells stained with 4’,6-diamidino-2-phenylindole (DAPI); images labeled with “2” show the normal view of cells, and images labeled with “3” are merged images of FITC stained cells, DAPI stained cells, and normal images, respectively. ( B ) Quantitative analysis of PPARγ gene expression in C3H10T1/2 cells treated with CMEs. Results are expressed as means ± standard deviation ( n = 3). Bars with different letters have mean values that are significantly different ( p < 0.05).

Article Snippet: The cells were then incubated with anti-PPARγ (81B8) rabbit monoclonal antibody (1:50) Cell Signalling Technology ( Danvers , MA, USA ) for one hour at ambient temperature, then washed gently with PBS and incubated in the dark with anti-rabbit IgG (Fab 2)–Alexa Fluor ® 488 (1:100; Cell Signalling Technology) for one hour.

Techniques: Immunofluorescence, Staining, Negative Control, Positive Control, Labeling, Gene Expression, Standard Deviation